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P2 appeared to reach a maximum comparatively early, at 16-20 hours; P3 did not reach a maximum until much later (44 hours), then declined. These proteins accounted for 60% of the virus tripartite genome. , 1980). Up to 11 virus-induced or virus-stimulated proteins were observed by using radioactive methionine labeling and polyacrylamide slab gel electrophoresis. Seven of these proteins appeared to be unique to infec­ tion, and four appeared to be strongly stimluated. Only one of the other proteins (MW 110,000) could be related to in vitro translation products of the CPMV RNAs.

However, with CMV in tomato, a different mechanism may be involved. Dodds (1982) found that prior inoculation with the protecting strain prevented both the accumulation and symptom ex­ pression of the challenge strain. Furthermore, the protection was re­ ciprocal and long lasting. This was the case even when a high-yielding CMV strain was used to challenge the protecting effect of the lowyielding strain. PROTOPLASTS AND PLANT VIRUSES 37 Otsuki and Takebe (1976a) studied double infection with common and tomato strains of TMV in tobacco protoplasts.

D. Synthesis of Virus-Related RNAs In the biology of the replication process, it may make little sense to separate the behavior and synthesis of the nucleic acids from that of the proteins involved. However, in terms of practical study, it may be desirable, because of the nature of the techniques used, the different timing and life spans of the two classes of viral components, to consider the synthesis of viral RNAs and proteins separately. Several viruses have been studied in recent years with regard to their RNA metabolism in inoculated protoplasts (Table V).

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